US3802909A - Bonding of organic materials to inorganic ...
Following ball milling, all unreacted enzyme was removed from the inorganic substrate by washing with isotonic saline. The washing procedure consisted of placing a 5 gm aliquot of the ball milled mix in a beaker with 200 ml saline, then agitating the mixture for 5 minutes, and then filtering with a Millipore filter.
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